Journal: bioRxiv

Article Title: Microbiome-derived hydroxyphenyl propanoates enhance antitumour immunity by potentiating gasdermin D activity in tumour-associated myeloid cells

doi: 10.64898/2026.04.23.720410

Figure Lengend Snippet: a, representation of the proteins with denaturing curves significantly altered (red) or unaltered (black) by 3,2-HPP treatment of THP1-Dual™ cells, using thermal proteome profiling (two combined experiments, proteomic coverage: 4301, NPARC test); b, protein-protein interaction network of proteins with denaturation curves altered by 3,2-HPP treatment (red) and the transcriptional regulators of 3, 2-HPP impacted genes in M3-9-M tumours (yellow); c, impact of 3,2-HPP treatment of THP1-Dual™ cells on the denaturation curve of GSDMD (two-combined experiments, NPARC test); d, NF-κB induction in THP1-Dual™ reporter cells treated with conditioned media harvested from WT or Gsdmd- KO THP1 cells treated with LPS ± HPP metabolites ± IL-1α and −1β neutralizing antibody (three combined experiments, one-way ANOVA with Bonferroni multiple comparison test); e-h, [secreted IL-1α and −1β] in conditioned media harvested from THP-1 WT or Gsdmd -KO cells, or human PBMCs, cultured for 16 hours in LPS ± HPPs (two combined experiments, one-way ANOVA with Bonferroni multiple comparison test); i-l, [secreted IL-1α and −1β] in conditioned media harvested from THP-1 WT vs. Gsdmd -KO cells, or human PBMCs, cultured for 16 hours in LPS and NG ± HPPs (two combined experiments, one-way ANOVA with Bonferroni multiple comparison test); m, western blot of THP-1 cells treated with LPS ± NG ± HPPs (representative of two experiments).

Article Snippet: To neutralize IL-1 receptor signalling, the following antibodies were used: anti-human IL-1α (1 μg/mL; clone 7D4; mabg-hil1a-3; InvivoGen), anti-human IL-1β (1 μg/mL; clone 4H5; mabg-hil1b-3; InvivoGen) and IgG1 isotype control (1 μg/mL; clone T8E5; mabg1-ctrlm; InvivoGen).

Techniques: Comparison, Cell Culture, Western Blot

Journal: Biomedical Reports

Article Title: Potential effect and mechanism of ultrashort wave therapy in mice with chronic rhinosinusitis

doi: 10.3892/br.2026.2109

Figure Lengend Snippet: Ultrashort wave therapy improves histopathology and inhibits apoptosis in sinus mucosal samples. (A) Hematoxylin and eosin staining revealed the pathological changes in sinus mucosal samples. (B and C) Apoptosis in sinus mucosa across different groups was assessed using TUNEL assay. Scale bars, 50 and 20 µm. ** P<0.01 and **** P<0.0001. N=6. CRS, chronic rhinosinusitis; ns, no significance.

Article Snippet: The sections were then blocked with 5% BSA (cat. no. ST023; Beyotime Institute of Biotechnology) at room temperature for 1 h. Next, the sections were incubated with primary antibodies against IFN-γ (cat. no. 15365-1-AP), IL-1α (cat. no. 83644-1-RR), TNF-α (cat. no. 17590-1-AP), and IL-10 (cat. no. 60269-1-Ig) (all diluted 1:50; Proteintech Group, Inc.).

Techniques: Histopathology, Staining, TUNEL Assay

Journal: Biomedical Reports

Article Title: Potential effect and mechanism of ultrashort wave therapy in mice with chronic rhinosinusitis

doi: 10.3892/br.2026.2109

Figure Lengend Snippet: Ultrashort wave therapy inhibits inflammatory responses in the sinus mucosal samples of mice with CRS. (A-E) Immunofluorescence was performed to assess the expression levels of IFN-γ, IL-1α, TNF-α, and IL-10 in sinus mucosal samples of mice with CRS. (F-I) The concentrations of IFN-γ, IL-1α, TNF-α, and IL-10 in nasal lavage fluid of mice with CRS were measured using ELISA. Scale bar, 100 µm. * P<0.05, ** P<0.01 and *** P<0.001. N=6. CRS, chronic rhinosinusitis; ns, no significance.

Article Snippet: The sections were then blocked with 5% BSA (cat. no. ST023; Beyotime Institute of Biotechnology) at room temperature for 1 h. Next, the sections were incubated with primary antibodies against IFN-γ (cat. no. 15365-1-AP), IL-1α (cat. no. 83644-1-RR), TNF-α (cat. no. 17590-1-AP), and IL-10 (cat. no. 60269-1-Ig) (all diluted 1:50; Proteintech Group, Inc.).

Techniques: Immunofluorescence, Expressing, Enzyme-linked Immunosorbent Assay