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anti-il-1α  (Bio X Cell)

 
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    Structured Review

    Bio X Cell anti-il-1α
    Anti Il 1α, supplied by Bio X Cell, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti-il-1α/product/Bio X Cell
    Average 90 stars, based on 1 article reviews
    anti-il-1α - by Bioz Stars, 2026-03
    90/100 stars

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    GO and KEGG pathway enrichment analyses of the putative targets of different components of Artemisia annua extract (AAE) for AD treatment ( a-e are Arteannuin B, Chlorogenic Acid, Scopolin, Vitexicarpin and Chrysoplenol D, respectively).
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    Image Search Results


    GO and KEGG pathway enrichment analyses of the putative targets of different components of Artemisia annua extract (AAE) for AD treatment ( a-e are Arteannuin B, Chlorogenic Acid, Scopolin, Vitexicarpin and Chrysoplenol D, respectively).

    Journal: Journal of Experimental Pharmacology

    Article Title: Artemisia annua Extract Ameliorates Atopic Dermatitis: Evidence from 3D Epidermal Model and Complementary in vitro Assays

    doi: 10.2147/JEP.S550568

    Figure Lengend Snippet: GO and KEGG pathway enrichment analyses of the putative targets of different components of Artemisia annua extract (AAE) for AD treatment ( a-e are Arteannuin B, Chlorogenic Acid, Scopolin, Vitexicarpin and Chrysoplenol D, respectively).

    Article Snippet: Protein concentration was determined by BCA assay, and cell lysates were resolved by SDS-PAGE on 4–12% gradient Bis-Tris gels (GenScript, M000138), transferred to PVDF membranes, and probed with antibodies against filaggrin (ThermoFisher, PA5-115235), IL-1α and TSLP (Cell Signaling Technology, 34819S and 97630S), p38 MAPK and p-p38 MAPK (Proteintech, 66234-1-Ig and 28796-1-AP), GAPDH (Cell Signaling Technology, 14C10), β-actin (Servicebio, GB15001-100), anti-rabbit IgG and anti-mouse IgG HRP-linked Antibody (Cell SignalingTechnology, 7074P2 and 7076P2).

    Techniques:

    Cytotoxicity of different AAE concentrations in keratinocytes cells. ( a ) Cell viabilities of keratinocytes after AAE (0.1–3%) treatment for 24 h; 0 group, treated with DMEM; AAE group, treated with AAE (0.1–3%); DMSO (positive control) group, treated with 10% DMSO (n=5). ( b ) Cell viabilities of keratinocytes after AAE (0.1–3%) treatment for 24 h in MβCD//IL-4/IL-13/IL-25-induced AD-like models (n=5) (* p < 0.05, significant difference; ** p < 0.01, highly significant difference; *** p < 0.001, extremely significant difference; **** p < 0.0001, ultra-significant difference).

    Journal: Journal of Experimental Pharmacology

    Article Title: Artemisia annua Extract Ameliorates Atopic Dermatitis: Evidence from 3D Epidermal Model and Complementary in vitro Assays

    doi: 10.2147/JEP.S550568

    Figure Lengend Snippet: Cytotoxicity of different AAE concentrations in keratinocytes cells. ( a ) Cell viabilities of keratinocytes after AAE (0.1–3%) treatment for 24 h; 0 group, treated with DMEM; AAE group, treated with AAE (0.1–3%); DMSO (positive control) group, treated with 10% DMSO (n=5). ( b ) Cell viabilities of keratinocytes after AAE (0.1–3%) treatment for 24 h in MβCD//IL-4/IL-13/IL-25-induced AD-like models (n=5) (* p < 0.05, significant difference; ** p < 0.01, highly significant difference; *** p < 0.001, extremely significant difference; **** p < 0.0001, ultra-significant difference).

    Article Snippet: Protein concentration was determined by BCA assay, and cell lysates were resolved by SDS-PAGE on 4–12% gradient Bis-Tris gels (GenScript, M000138), transferred to PVDF membranes, and probed with antibodies against filaggrin (ThermoFisher, PA5-115235), IL-1α and TSLP (Cell Signaling Technology, 34819S and 97630S), p38 MAPK and p-p38 MAPK (Proteintech, 66234-1-Ig and 28796-1-AP), GAPDH (Cell Signaling Technology, 14C10), β-actin (Servicebio, GB15001-100), anti-rabbit IgG and anti-mouse IgG HRP-linked Antibody (Cell SignalingTechnology, 7074P2 and 7076P2).

    Techniques: Positive Control

    MβCD/IL-mix-induced AD-like phenotypes and expression of related genes in 3D epidermal models. ( a ) Histological sections of 3D models treated with different concentrations of MβCD/IL-4/IL-13/IL-25. 25: MβCD (7.5 mM), IL-4 (25 ng/mL), IL-13 (25 ng/mL), IL-25 (10 ng/mL); 50: MβCD (7.5 mM), IL-4 (50 ng/mL), IL-13 (50 ng/mL), IL-25 (20 ng/mL); 100: MβCD (7.5 mM), IL-4 (100 ng/mL), IL-13 (100 ng/mL), IL-25 (40 ng/mL). Scale bar 50 µm (n=3). ( b ) qPCR analysis (n=4) (* p < 0.05, ** p < 0.01, **** p < 0.0001, n.s., not significant). ( c ) Cytokine array TSLP and IL-1α analysis (n=3) (** p < 0.01, **** p < 0.0001).

    Journal: Journal of Experimental Pharmacology

    Article Title: Artemisia annua Extract Ameliorates Atopic Dermatitis: Evidence from 3D Epidermal Model and Complementary in vitro Assays

    doi: 10.2147/JEP.S550568

    Figure Lengend Snippet: MβCD/IL-mix-induced AD-like phenotypes and expression of related genes in 3D epidermal models. ( a ) Histological sections of 3D models treated with different concentrations of MβCD/IL-4/IL-13/IL-25. 25: MβCD (7.5 mM), IL-4 (25 ng/mL), IL-13 (25 ng/mL), IL-25 (10 ng/mL); 50: MβCD (7.5 mM), IL-4 (50 ng/mL), IL-13 (50 ng/mL), IL-25 (20 ng/mL); 100: MβCD (7.5 mM), IL-4 (100 ng/mL), IL-13 (100 ng/mL), IL-25 (40 ng/mL). Scale bar 50 µm (n=3). ( b ) qPCR analysis (n=4) (* p < 0.05, ** p < 0.01, **** p < 0.0001, n.s., not significant). ( c ) Cytokine array TSLP and IL-1α analysis (n=3) (** p < 0.01, **** p < 0.0001).

    Article Snippet: Protein concentration was determined by BCA assay, and cell lysates were resolved by SDS-PAGE on 4–12% gradient Bis-Tris gels (GenScript, M000138), transferred to PVDF membranes, and probed with antibodies against filaggrin (ThermoFisher, PA5-115235), IL-1α and TSLP (Cell Signaling Technology, 34819S and 97630S), p38 MAPK and p-p38 MAPK (Proteintech, 66234-1-Ig and 28796-1-AP), GAPDH (Cell Signaling Technology, 14C10), β-actin (Servicebio, GB15001-100), anti-rabbit IgG and anti-mouse IgG HRP-linked Antibody (Cell SignalingTechnology, 7074P2 and 7076P2).

    Techniques: Expressing

    AAE partially improved the AD-related cytokine expression in AD-like skin equivalent model. ( a ) Cytokine array TSLP, IL-1α, IL-6 and IL-8 analysis (n=3) (** p < 0.01, *** p < 0.001, **** p < 0.0001, n.s., not significant). ( b and c ) Protein expressions of IL-1α and TSLP determined by Western blot (n=4) (* p < 0.05, ** p < 0.01, *** p < 0.001).

    Journal: Journal of Experimental Pharmacology

    Article Title: Artemisia annua Extract Ameliorates Atopic Dermatitis: Evidence from 3D Epidermal Model and Complementary in vitro Assays

    doi: 10.2147/JEP.S550568

    Figure Lengend Snippet: AAE partially improved the AD-related cytokine expression in AD-like skin equivalent model. ( a ) Cytokine array TSLP, IL-1α, IL-6 and IL-8 analysis (n=3) (** p < 0.01, *** p < 0.001, **** p < 0.0001, n.s., not significant). ( b and c ) Protein expressions of IL-1α and TSLP determined by Western blot (n=4) (* p < 0.05, ** p < 0.01, *** p < 0.001).

    Article Snippet: Protein concentration was determined by BCA assay, and cell lysates were resolved by SDS-PAGE on 4–12% gradient Bis-Tris gels (GenScript, M000138), transferred to PVDF membranes, and probed with antibodies against filaggrin (ThermoFisher, PA5-115235), IL-1α and TSLP (Cell Signaling Technology, 34819S and 97630S), p38 MAPK and p-p38 MAPK (Proteintech, 66234-1-Ig and 28796-1-AP), GAPDH (Cell Signaling Technology, 14C10), β-actin (Servicebio, GB15001-100), anti-rabbit IgG and anti-mouse IgG HRP-linked Antibody (Cell SignalingTechnology, 7074P2 and 7076P2).

    Techniques: Expressing, Western Blot